Proetin expression in insect cells - (Sep/08/2005 )
Does anyone have a suggestion as to where I can find good information (from basics to the complex) on protein expression in insect cells. I have used several systems with varied results and different problems. Each time I call a company's tech support, they give me a brief discription of how there system works. I would like a detailed description of transient systems, stable integration systems, and viral systems, so I can try and design or optimize my present practices. I need to educate my self on how and why these company's design the systems the way they do.
Baculoviral systems would be a good place to start. I'd search a graduate level molecular biology textbook or pubMed for info.