Whole Cell PCR from BacteriaProtocols? - (May/25/2001 )
You can use bacterial culture directly as template to do PCR, I never had problem before with that.
Does anybody know of some good protocols for direct or whole cell PCR from bacteria? i've heard of doing this without having to pay for all the miniprep kits, (for microarraying, 40,000 times is a lot of kits) but wondering how clean a product (of course) we would get from this, how much junk is going to be in with the PCR?
and ....i use direct colony pick-up method!colony was picked by tip or any other sterized tip-like something.and transfer normal PCR conditioning tube..and then pre-denaturing time needed more 3min.
What I did is denaturing the cells for 10 mins, and then add the PCR cocktail and run the pcr.
Just swipe a colony from an agar plate. Stick into ddH2o. Boil for less than a minute. Stick it in your PCR cocktail. It works beautifully.