siRNA in non-adherent cell lines? - Tips please (Mar/03/2006 )
I'm going to try knockdown in a non-adherent cell line, I've had success with oligofectamine in adherent cell lines but wanted to ask if anyone had managed using oligofectamine for transfecting non-adherent cell lines? Or can anyone recommend a better method?
i use lipofectamine foer adherent cells and for non adherent ones. Protocol is the same regarding time of tranfection and ratio DNA/lipocomplex. I use twice DNA quantity per same amount of cells.
Cheers, I'll try that one out
some threads may be useful too...
In search you type suspension cells. I've listed some..
For that reason, we turned to eletroporation-Amaxa Nucleofector, after optimization, for early passage Jurkat cells (<10), we could get >90% efficiency.
That's a great help I'll look into trying out some of those methods.