Does immortalization affect non-dividing short-living cells? - (Sep/15/2008 )
to analyze native short-living cells (f.i. enterocytes, some exocrine cells etc.) in vitro, one would try to prolong survival after isolating these cells; as these cells are cell cycle arrested, immortalization to my point of view is useless but I am not sure; what do you think, please?
-The Bearer-
Immortalisation means that they continue dividing indefinitely. If this would change the physiological characteristics of the cells then it is probably not a good thing to do. Immortalisation may also be quite difficult if the cells are in S phase (unless you are infecting with a virus to force them into cycling again).
-bob1-
QUOTE (bob1 @ Sep 15 2008, 04:16 PM)
Immortalisation means that they continue dividing indefinitely. If this would change the physiological characteristics of the cells then it is probably not a good thing to do. Immortalisation may also be quite difficult if the cells are in S phase (unless you are infecting with a virus to force them into cycling again).
nice answer, thanks
; I know immortalization with SV40; is this such a virus to relase cells form cell cycle arrest? which other (biotechnologically used) virus´make this? please advice
-The Bearer-
QUOTE (The Bearer @ Sep 16 2008, 01:21 PM)
QUOTE (bob1 @ Sep 15 2008, 04:16 PM)
Immortalisation means that they continue dividing indefinitely. If this would change the physiological characteristics of the cells then it is probably not a good thing to do. Immortalisation may also be quite difficult if the cells are in S phase (unless you are infecting with a virus to force them into cycling again).
nice answer, thanks
; I know immortalization with SV40; is this such a virus to relase cells form cell cycle arrest? which other (biotechnologically used) virus´make this? please advice
Any of the DNA tumour viruses will be capable of it, but some are better than others. SV40 is the commonly used one, but adenoviruses are also used.
-bob1-