siRNA transfection of MIN6 cells - (May/17/2006 )
Hi Everyone,
I am trying to do siRNA transfections on MIN6 cells. I am using neofix as transfecting reagent. I am still optimising the process but the results are not good.
Can any one could help me out as to which transfecting reagent to use or any other protocol.
Thanks and Regards
-sweta rani-
Hi Everyone,
I am trying to do siRNA transfections on MIN6 cells. I am using neofix as transfecting reagent. I am still optimising the process but the results are not good.
Can any one could help me out as to which transfecting reagent to use or any other protocol.
Thanks and Regards
-sweta rani-
QUOTE (sweta rani @ May 17 2006, 07:51 AM)
Hi Everyone,
I am trying to do siRNA transfections on MIN6 cells. I am using neofix as transfecting reagent. I am still optimising the process but the results are not good.
Can any one could help me out as to which transfecting reagent to use or any other protocol.
Thanks and Regards
I am trying to do siRNA transfections on MIN6 cells. I am using neofix as transfecting reagent. I am still optimising the process but the results are not good.
Can any one could help me out as to which transfecting reagent to use or any other protocol.
Thanks and Regards
We have great success (~60-70%) using Gencarrier-2 on MIN6 mouse beta-cell line with FITC-siRNA duplex (27-mer). No or little cell loss.
-fair view-