aza treatment of D283 cells - aza treatment of D283 cells (Jan/22/2007 )

hi,
i want to treat my medulloblastoma cells, D283, but I think that my cells dye then 48 hours adding aza from one pilot treatment...
UMMM...i don't know how cells I must plate on 100mm plate..i consider about 70% confluence..is it right???
how aza concentration?
i want use 5uM...
i want treat my cells for 5 days replacement every days medium and adding fresh aza.. what do you think?
thanks so much

aza will kill cells within a day. You need to set up a titration of concentrations to see how your cells handle it.