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aza treatment of D283 cells - aza treatment of D283 cells (Jan/22/2007 )

hi, rolleyes.gif
i want to treat my medulloblastoma cells, D283, but I think that my cells dye then 48 hours adding aza from one pilot treatment...
UMMM...i don't know how cells I must plate on 100mm plate..i consider about 70% it right???
how aza concentration?
i want use 5uM...
i want treat my cells for 5 days replacement every days medium and adding fresh aza.. unsure.gif what do you think?
thanks so much


aza will kill cells within a day. You need to set up a titration of concentrations to see how your cells handle it.