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Visualizing Cell Pellet - (Nov/13/2004 )

Hi All,

I am currently working with very small amounts of primary cells (>10,000 cells), and I seem to be losing my cell pellet quite often. I would therefore like to know if anybody know of a method to visualize my cell pellet (e.g. similar to glycogen for DNA/RNA)? I would assume that a positive charged polymer would do the trick, but it would most likely inhibit downstream applications.

Also what is the max rcf for spinning lymphocytes?

Thanks a bunch cool.gif


What kind of tubes do you use for the centrifugation? If you use a round-bottom tube such as the 2ml one you may not see the pellet easily. So use small v bottom tubes.