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HEK293 cell culture protocol - (Sep/14/2001 )

Who can tell me the protocol of how to culture the HEK293 in detail? Thanks!


I just got some HEK293's from CloneTech for use as packaging cell line.I'm culturing them in DMEM(hi glucose), 10%FBS, pen/strep and plated themon Biocoat Collagen 1 plates. They seem to be growing fine.After 2 months, they recommend re-selecting the cells in zeocin and puromycin for couple weeks.

- Ryan