Tween 20 and Cells - Question - (May/19/2007 )

ive been using tween 20 (0.05%) to permeabilize some suspension cells. i notice that there is a considerable difference in the appearance of the cells before and after. before treatment they are much easier to image but after they look flatter and more faint - why is this?

thanks so much

The answer is in your question:P

Tween is a chemical that permeabilizes membranes. The integrity of the cells is therefore expected to differ when treated with Tween.