Glycerol gradients - glycerol gradients for cell extract fractionation (Oct/01/2005 )
Hi everybody! I would like to know if anybody fractionates protein complexes from whole cell extracts on glycerol gradients. How are they prepared and run? I would like to have a try on it and have never done.
Thanks a lot
The main question I've got about glycerol gradients preparation is wether detergent inclusion in the solvent of the gradient can give problems or not. My sample, a whole cell extract, contains .5%NP40.
I have the same problem as you. Did not find any useful protocol at all?