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Glycerol gradients - glycerol gradients for cell extract fractionation (Oct/01/2005 )

Hi everybody! I would like to know if anybody fractionates protein complexes from whole cell extracts on glycerol gradients. How are they prepared and run? I would like to have a try on it and have never done.

Thanks a lot

Miguel

-miguelon-

The main question I've got about glycerol gradients preparation is wether detergent inclusion in the solvent of the gradient can give problems or not. My sample, a whole cell extract, contains .5%NP40.

Thanks everybody

-miguelon-

QUOTE (miguelon @ Oct 7 2005, 10:23 AM)
The main question I've got about glycerol gradients preparation is wether detergent inclusion in the solvent of the gradient can give problems or not. My sample, a whole cell extract, contains .5%NP40.

Thanks everybody

I have the same problem as you. Did not find any useful protocol at all?
God help.

-cathy-