can arginine be used as nitric oxide donor compound in cell culture. - kinly help!!!!!!!! (Dec/20/2006 )
hi everyone,
i want to know whether arginine be used as NO donor compound in cell culture. and if yes send me some literature, if possible.
i will be very thankful.
-pBS-
Dear pBS,
Arginine is the amino acid (substrate) required by Nitric Oxide Synthase to generate NO. It is therefore NOT an NO donor like SNAP or DETA NO i.e. Nitric oxide will not be liberated from arginine in aqueous solution.
What sort of experiments are you interested in ?
-Rhombus-
QUOTE (Rhombus @ Dec 21 2006, 03:35 AM)
Dear pBS,
Arginine is the amino acid (substrate) required by Nitric Oxide Synthase to generate NO. It is therefore NOT an NO donor like SNAP or DETA NO i.e. Nitric oxide will not be liberated from arginine in aqueous solution.
What sort of experiments are you interested in ?
Arginine is the amino acid (substrate) required by Nitric Oxide Synthase to generate NO. It is therefore NOT an NO donor like SNAP or DETA NO i.e. Nitric oxide will not be liberated from arginine in aqueous solution.
What sort of experiments are you interested in ?
actually i saw in few paper that arginine is acting as a NO donor in in vivo experiments. and i am looking forward for the same in in vitro condition.
that why i put this question on bioforum.
have you heard paper related to this.
if yes please send me the literature.
thanx
-pBS-
have no idea but i agree that it is not an NO donor. be careful some NO donors should be modified in order to enter the cell.
-Kathy-