Need protocol for mouse ES cell stable transfection - (May/29/2006 )
Hi,everybody,I will stably deliver a gene into mES cells,which is new to me, Does anybody has some suugestions or experience (protocol )in this work?
Thanks in advance!
-JerryLee-
For ES cells, our experience is that for transient transfection use Gencarrier-2 which showed the best outcome (efficiency over toxicity); for stable transfection, use lentiviral vector (Invitrogen).
-postdoc2130-