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Doesn't anyone use roller bottles to culture cells in anymore? - Let's compare notes. (Nov/16/2007 )

I know everyone uses roller bottles. What method of cell adhesion and removal do you use to optimize cell yields using your bottles?
rolleyes.gif

-dave2-

QUOTE (dave2 @ Nov 16 2007, 08:50 AM)
I know everyone uses roller bottles. What method of cell adhesion and removal do you use to optimize cell yields using your bottles?
rolleyes.gif


Good question dave2. I see that you have once again stumped everyone. There are many types of polystyrene substrates such as both film and hardened plastic that are corona and microwaved treated for a negitive charge (needs a bridge). As for removing the cells, I hope someone has a good method to share with us.

-dave2-

did you compare notes:) I think you thin loudly:)
best

-mhmtcn-