Any effect of different culture dishe size on cell? - (Aug/20/2006 )

hi


can anybudy tell me

is there any effect of using different size of dishes for culturing

hi,
density of cells is very important.

regards
payeli.

hi
u have to adjust ur density according to the size of ur plate and also the kind of expierement u are doing can determine which dish u will use , dont forget also that lareg dish means more media.

hi,

i wish this pdf will be usefull...

Seb

Hi there!

where did you get the table from? It is great!

Stardust

This pdf is really useful. Thanks! However, there is no numbers for 96-well plate. Can anyone help?

For 96 well plates,

surface area- 0.3 cm2
vol. of media- 0.1-0.2 ml
amount for trypsin - 50ul

i dont know abt cell densities.

A correction:

I think the number listed for 100 mm dish is wrong. According to my measurement, the diameter for 100 mm dish is only 8.5 cm, not 10 cm the name indicated. This translates to a surface area of ~ 5671 mm^2, or ~75% of the surface of listed. So multiply 0.75 to the numbers for 100 mm dish everyone!!!

I become suspicious when I saw the numbers of 100 mm dish and T75 flask. We all know a T75 is bigger than 100 mm dish, right?


PS. I would suggest keeping the last two items the same. i.e. 3 ml for trypsin-EDTA and 10 ml for growth.