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Will low concentration of ethanol affect cell line? - (Feb/13/2006 )

I need to dilute dexamethasone powder in ethanol then add into cell medium. The final cencentration of ethanol is low. But will even low concentration of ethanol affect cell?

-macrosky-

Hi

How low? It also depends on the cell line, some are more sensitive than others. I would suggest adding it dropwise into the medium with mixing between drops.

I can't remember the tolerances, but I think most cells will tolerate less than 0.1% OK, though I could be an order of magnitude out there.

Good luck
Bob

-bob1-