basic techniques for cell treatment - (Jun/20/2006 )
Hi,
I am a newer in bench work with cell. Sometimes I want to treat cells by chemicals, but I don't know where I should treat them, such as in dishes, in plates, or in tubes. Also I don't know how much cells are needed for the specific vessels and for specific aims, such as western blot or other experiments. I tried to find similar experiments from other papers, but in method part there is little detailed information. I don't know whether you guys have the similar problem as me? Where did you guys get this useful information?
Thank you very much.
Is there not a senior or a labmate who can train you? tissue culture is really not something that can be self-taught, it's something that needs to be learned at the bench with visual example
aimikins is right. Its better to ask some senior labmate to teach u the basics else if u try to do this urself, u could actually do more harm to than gain knowledge.
Yes, you are right, but the problem is that there is no senior labmate in my lab. My boss don't have enough grant to support.
I don't know what I can do. I don't know what I can do.is there someone from another lab who could teach you?
but the problem is that there is no senior labmate in my lab. My boss don't have enough grant to support.
I don't know what I can do.is there someone from another lab who could teach you?
That's what I had to do when I started in my lab. I had some tissue culture experience, but none with the cells we were working with and no one in my area knew very much about tissue culture. So I went to two different labs one at a different institution where I knew the lab manager and to the tissue culture lab in my department.
Most people are willing to help you learn the basics. I would look for a person from another lab and maybe send them an email to see if they can help you. Does the institution you are at have a tissue/cell culture lab? That would be a great place to start asking questions.
Most people are willing to help you learn the basics. I would look for a person from another lab and maybe send them an email to see if they can help you. Does the institution you are at have a tissue/cell culture lab? That would be a great place to start asking questions.
I think this is a very sensible idea. You need somebody experienced to show you the basics. If you haven't got anybody in your own lab you need to seek help elsewhere. If you're told they can't help because they're too busy, see if you can get your foot in the door by asking if you can just watch and ask a few questions along the way.