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aml-2 cells - contamination in aml-2 cells (Nov/21/2004 )

my aml-cells in the past grew very well but now i am facing problems in its growth. i can observe the shrunken cells, only 25% refractile cells and innumerous black dots like structures in the culture medium. how can i solve this problem????

-puspa raj pandey-

Its better to discard these cells as they look to b contaminated. Revive a new stock of cells. Better not to take chances in cell culture by continuing with contaminated cultures!!!