Hi, anyone culture SKOV3 cells in vivo?
what should they look like under the microscope? should they look bright and round or dark star-shaped?

i culture some skov3 from frozen stock yesterday but they don't seem happy today and mostly are dead, don't attach to the bottom of flask.


seeking advice~~~thx~~~~~~

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Mine look star-shaped. I just split them today but I can give more detail tomorrow if you need. You may have either bad medium or a bad stock. What medium are you using?

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Thanks for your info! It's really helpful!

The media I used was RPMI 1640 HEPES modification from sigma, supplemented with glutamin, P/S and FCS.

I took another SKOV3 stock today and cultured. After a few hours, there're some cells looked star-shaped and some were bright and round-shaped. But I'm not sure which ones were dead or alive. I cultured LoVo cells before, and they looked really bright and sphere under the microscope. Guess there must be variant morphology between these 2 cell lines

waiting for your further details.. thanks!

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Sounds about right. The star shaped are cells that are alive and attached; the round shaped ones may simply be cells that are still attaching. Overall it sounds as if you are OK now and probably just had a bad stock the first time.

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