HELP: transfection reagent for BJ cells - (Dec/10/2007 )
a neighbouring lab used FugeneHD with 20% transfection efficacy. You can also try Amaxa nucleofection. Personally I found that BJs are very easy to infect using retroviruses (infection efficacy about 70-80%). However, I am sure you know, BJs have a limited proliferation capacity. When they get older they are much harder to infect. Make sure you have young BJs (population doubling <30).