E.coli TOP10 comp cells T1 Phage resistant? - (Sep/13/2007 )
Hello, I am looking for E.coli TOP10 comp cells, T1 Phage resistant for protein expression.
Can anyone recommend a strain please or any suppliers?
Top10 cells are not usually used for protein expression. They are lon+ and contain all of the native proteases, which are largely missing from BL21 and similar strains designed for expression. See http://openwetware.org/wiki/E._coli_genotypes