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mouse B cells - (Mar/16/2006 )

I am trying to design mouse experiements involing the isolation of B cells and subsequent protein and RNA isolation. I need to know the approximate size of a mouse spleen, approximately how many B cells are in one spleen, do you think I can get enough protein or RNA out of the B cells from one spleen, or do I need to pool from a number of mice? I cannot seem to find this info anywhere. All the papers I have read make it seem like one mouse spleen is enough for protein and a second for RNA, but I am not sure.




of course one spleen is sufficient for RNA or protein extraction, it contains approx. 50e6 B after magnetic bead isolation