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SF9 cells - (Feb/13/2005 )

Does anyone have any idea which antibiotic shoud we use for SF9 cells ? Moreover which protocol is better for transfection of SF9 cells using calcium chloride or lipfectin?


I use pen/strep at the concentrations one would use in general cell culture. For the transfection I use cellfectin with good results. havent tried any other method yet.


i use blasticidin and cellfectin on a similar cell line