Stable cell trasfection questions... - Integration of plasmid... (Dec/06/2006 )
Some basic questions on stable cell transfection... In which moment the plasmid is incorporated to cell DNA? What enzimes participate? Is it a kind of recombination process? The site of incorporation... is it random?
Thanks a lot.
u will find answers to ur question here
i found this article s, i thought it might help.
Excellent! Thanks a lot, spanishflower! For all your replies too .
I have the same problem for selecting a stable clone from primary neural stem cell.
I tried to select the clone from neural stem cell that expressing my gene but it lost the
differentiating ability soon after the selection. I have tried many times as well as got the
same results. It was really tried to pick the spheres from the bulk cultures under
Transfection reagent : DMRIEC & Lipofectamine 2000