Cell Differentiation - Time periods and environment for proper cell differentiation (Aug/03/2006 )

I am developing a protocol that involves both Vitamin D3 differentiation and PMA differentiation. How long do each of these two agents need to induce differentiation?

-prog23-

QUOTE (prog23 @ Aug 3 2006, 07:09 PM)

I am developing a protocol that involves both Vitamin D3 differentiation and PMA differentiation. How long do each of these two agents need to induce differentiation?

What cells/cell line are you using?

For THP-1 cells - Vit D3 72h, PMA 24h

-bxj119-