MTS assay of MCF7 and MDA-MB-453 cells - (Aug/27/2006 )
I am working on MTS assay of MCF-7 and MDA-MB-453 cells. Basically, what I do is transfecting these cells with siRNA, 24hours later assaying their viability. During my early trials I found that the medium of each cell line kind of react with the MTS assay reagent (Promega). I am wondering if the medium can be replaced with Opti-MEM just before MTS assay, followed by addition of MTS reagent and incubation for 1-4 hours. Will that affect the viability of those cells? If this method is ok, which incubator should I put the MDA-MB-453 cells in? with their original medium (Leibovitz's L-15), they need to be in a no-CO2 incubator. With Opti-MEM, they should be in a 5% CO2 incubator, right?
I am new with this assay, so I have so many questions. Thanks in advance to anyone who could help me!
I couldn't understand how "the medium of each cell line kind of react with the MTS assay reagent." Can you tell they reacted please?