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Cell adhesion assays/protocols - Any suggestions? (Jul/09/2005 )

I want to study the adhesion of one population of cells (normally suspension) to another (adherent monolayer population). What assays can I use to evaluate adhesion? I will be doing several manipulations of the suspension cells and want to test adhesion before (usually minimal) and after (potentially extensive).

Thanks in advance for any ideas.

-MaximinaNYC-

bump, any idea?

-MaximinaNYC-

QUOTE (MaximinaNYC @ Jul 16 2005, 03:46 AM)
bump, any idea?



Hi,
you could look cytoskeleton organization by rhodamine-phalloidin, if your cells have different morphology and you can distinguish them. Sometimes it is very informative method

good results)))))

-shanru-

Thank you I will try it but what would you expect to see with such a method on suspension cells. Can I label them prior to adhesion so to distinguish the cell populations?

-MaximinaNYC-

A classic adhesion assay involves labelling your suspension cells with Chromium 51 allowing them to adhere, wash off the non adherent with PBS then solubulise and count in a scintilation counter. This method has been very widely used to look at heamopoetic cells adhereing to fibroblasts or matrix proteins and can be performed in 96 well plates.

-ajames-

Thank you ajames, this is exactly what I need to point me in the right direction. THANKS! biggrin.gif

-MaximinaNYC-