Protocol Online logo
Top : Forum Archives: : Cell Biology

tgf-beta in cell culture - (Feb/14/2005 )

I'm looking for the right concentrations of TGF-beta to use in human cell cultures in order to produce a normal non-pathogenic response.

All suggestions are welcome!


What cells are you working with?

Try lots of concentrations. I have used from 1 pg/ml to 10 ng/ml on bone marrow endothelial cells (grown in collagen) and have gotten interesting results depending on the concentration. High concentration will probably just kill your cells, unless they are resistant to TGFbeta signaling. I had interesting results in the range of 1 - 100 pg/ml. For your study, I'd say try 1, 10, and 100pg/ml and 1 and 10 ng/ml and then use the concentrations that seem best.