Red Blood Cell percentage in human spleen - (Jun/06/2007 )
Does anyone know the percentage of RBC in human splenocytes (not spleen blood but single suspension cells of lysised spleen) ? And what's the percentage of RBC in normal human peripheral blood? Thanks!
Why do you want to know the percentage of RBCs in spleens? RBCs would be zero if you can remove all blood from spleen. Thus, I think it depends on how much blood is in spleen.
I'm using a kit from Stem cell to negatively select human B cells. This kit utilizes the tetrimeric-antibody-complex (TAC) to crosslink non-B cells to multiple RBC to form a big complex so that the density of these non-B cells will be increased to that of RBC, they then can be separated from enriched B cells during the Percoll gradient. The kit works very well and it's much cheaper than the similar product.
So you see, you cannot get rid of the RBC in this case, and I want to know the yield of B cells. The problem is that I cannot distinguish RBC and other immune cells with just trypan blue counting... I guess the easiest way is take some of the whole cells, lysis the RBC and count the rest cells.
BTW, spleen is considered as a blood reservoir organ, RBCs are distributed all over the organ.
you can stain leukocytes by Turk solution.
the word percentage in human peripheral blood that I usually hear, is used in Hb (the unit used is g/dl or g%).
percentage of RBC in human splenocytes? sorry I can't answer that. All I know that red blood cells are stored in the sinusoids of the spleen, and (I think) not in the cells. The RBC in the spleen sinusoids will be extracted if there is a bleeding, thus will help to increase the Hb.