Cell Biology - protocol for reactive oxgyen species (Aug/16/2004 )

I am using primary thymocyte cell culture and I find a decrease in ROS in the normal cell population during the first 30 min. I am using DCFH - DA dye ( 100 um) ,15 min prior to cell acquisition by flow cytometry for 30 min.
Please suggest an explanation for such a decrease. I expected no change.

Hi
First off all u may run ur system by using ROS generating system.
Kishore=======