TEM of langerhans cells - (Mar/09/2006 )
Dear bioforum members,
i have to perform transmission electron microscopy of mouse epidermal langerhans cells
suspension. can anybody suggest me appropriate stain and protocol to do this
without using monoclonal antibody.
vivek
-vmsy2k-
QUOTE (vmsy2k @ Mar 9 2006, 07:17 AM)
Dear bioforum members,
i have to perform transmission electron microscopy of mouse epidermal langerhans cells
suspension. can anybody suggest me appropriate stain and protocol to do this
without using monoclonal antibody.
vivek
i have to perform transmission electron microscopy of mouse epidermal langerhans cells
suspension. can anybody suggest me appropriate stain and protocol to do this
without using monoclonal antibody.
vivek
Hi Vivek,
I have used the following protocol for Cancer cells. You can use this one. Just click the link below.
http://www.ihcworld.com/_protocols/em/em_routine_cell.htm
Thanks
Deb
-debarshir-