TSA Concentrations for Pancreatic Cell lines - (Jul/29/2005 )

Hi there!

I've just started in the field and am currently researching pancreatic cells and their methylation status. In this course I'm desperately trying to find any data on TSA experiments, especially TSA concentrations (dose response curve), with the following cell lines:

HS766T
SV8686
BxPC3
Capan2
MiaPaca2
Panc1
Capan1
Mpanc 96
Hpac
PL45
CoCa retro
CoCa C1199

If you by chance have some interesting knowledge, database, advice, just anything, I would be very grateful!

Thanks a lot!

Hi,

TSA titration curves, like 5-aza-dC curves are not all available for all cell lines.

TSA would be an indirect influence in DNA methylation as it is a histone inhibitor.

My experience with looking fir 5-aza-dC titrations, these can vary from paper to paper using the same cell line!! if it is feasible, you would need to perform titration curves and treatment times yourself, then you would be sure of it as opposed to trusting a published protocol as some, not all may be inaccurate or just wrong!!

We have performed a titration of TSA using CHO-K1 somatic cell hybrids and have been using 75nM overnight treatment to give a chromatin effect without killing the cells completely.

good luck!!

Thanks for your reply!
We will be using 3 different concentrations for now, skipping the dose response curves and treating cells with 0.1, 0.5, and 1 ng/ml concentrations.

I used 400 nM TSA 16-18 h for the treatment of the following PCa cell lines:

IMIM PC2
SKPC1
PT45P1
MiaPaCa2

The experiment worked out pretty well.