how many cells in a mouse spleen - (Dec/14/2007 )
Does anyone have a good estimate or a credible source of estimate for how many total cells there are in a spleen? I have searched all morning and don't have an answer. Thanks.
have you contacted Jackson? they have some of that data available online. I think it may be strain-dependent as well, in some cases.
if you can't find a link on their site, I would call them. in my exp, their customer support is usually great
the number of cells in the spleen would depend on the strain
but for a rought estimate it can be around 80-90x10^6 cells as i count the cells when i harvest the spleen from balb/c mice for hybridoma
It will slightly vary from strain to strain probably, but in un-manipulated mice ( of the A.SW and C57BL/6 strains) I find between 35 - 60 million following RBC lysis.
I'm agree with Leeraram.
when i harvest the spleen from balb/c mice for hybridoma, after hyperimmunization, the number of cells in the spleen can be around 80-90x10^6 cells after erithrocytes lysis.
thanks a lot guys, my boss wants me to get an estimate as well as the source. Is Jackson a creditable source or is there any papers out there that has exp. or estimate on this?