Isolation of cell membrane - (May/04/2001 )
Can anybody recommendate a good method for isolation of cell membrane from cell line? Thanks a lot. Yaoting
YES. I AM WORKING ON SIGNALING PATHWAY VIA CELL MEM. AND THE METHOD WE ARE USING IS WORK WELL IN MY HAND.PLEASE LET ME KNOW YOUR EMAIL ADDRESS SO THAT I WILL LIKE TO SEND MY METHOD.
Would this method also work for isolating cell surface/membrane associated antigens and if so, could you explain the procedure to me?
Hi,Do you think your method will work also for partially purifying receptors from membrane?If so please forward it to me.Yael
Would you please give me a protocol to prepare the bacterial membranes for the western blot. The membranes I prepared didn't dissolve properly in Tris buffer.
Thanks, Xueyan Duan
can you mail me the method too. haviong a tough time isolating membrane from cell line... because of quantity problems. Mine emal ID is firstname.lastname@example.org.