Remove BSA from cell culture medium - (Jan/25/2007 )
Hi there,
I'm trying to see a protein my cells are secreting in the cell culture medium on SDS PAGE gels...However all I see ist a big "blob" of albumin (I'm using serum reduced medium with BSA as a component left over in the medium) and there is no way of doing 100 % serum free culture. Does anyone know a method to remove the BSA before running the gel? Any beads, columns, ...? I found some things for removal of HSA but not BSA...
Thanks Stardust
most of those things you found that will remove hsa will also remove bsa. they also tend to remove some other proteins so be careful.
Hi!
Try this one
Amersham old product
HiTrap™ Blue HP Columns
Prepacked with Blue Sepharose™ High Performance for purifying albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons, and related proteins.
Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked agarose medium and is stable over a pH range of 4-12 for use and 3-13 for cleaning.
Try this one
Amersham old product
HiTrap™ Blue HP Columns
Prepacked with Blue Sepharose™ High Performance for purifying albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons, and related proteins.
Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked agarose medium and is stable over a pH range of 4-12 for use and 3-13 for cleaning.
problem is stardust wants to remove albumin, not purify it and, as the description you give says, it will bind many other proteins. we had tried it for the same purpose and found that it bound too many other proteins (to varying degrees) to suit our purpose.
I have not tested it myself, but this sounds like it may help you. Maybe someone who has used can tell you more if it works as it claims.
http://www.laubscherlabs.com/Presentation/...n%20Removal.pdf
thanks, the link is very helpful!