yeast cell cycle and temperature - FACS profile at 25 and 36 (Jun/04/2008 )
Hello. Does anyone know if the FACS profile for asynchronous yeast cells looks different at 25C vs 36C? For example, do you see a higher accumulation in G1 at 25 than you would at 36?
Also has anyone else noticed the temporary arrest in G1 after galactose addition to asynch cells growing in YPR?
-sumogirlie-
QUOTE (sumogirlie @ Jun 4 2008, 10:41 AM)
Hello. Does anyone know if the FACS profile for asynchronous yeast cells looks different at 25C vs 36C? For example, do you see a higher accumulation in G1 at 25 than you would at 36?
Also has anyone else noticed the temporary arrest in G1 after galactose addition to asynch cells growing in YPR?
Also has anyone else noticed the temporary arrest in G1 after galactose addition to asynch cells growing in YPR?
You may find answers in the first link here:
http://search.vadlo.com/b/q?sn=158621799&a...ells+&rel=0
..
-cellcounter-