How to make the neural stem cell differentiate ? - Neurospheres from mouse E14 (Mar/29/2006 )
Urgent help !! I bought the mouse NSC from stemcell technologies
but it didn't undergo differentiation , although replaced by the differentiation
medium. The cells didn't attached to the disk and remained expand as sphere. I
didn't know what was happened in my experiment. I am sure I have coated the ornithine
at 15 micro-gram/ml. Could anyone give me some advice ? Help !! I have no time ...
I found that it was very difficult for handling the neural stem cell undergo differentiation
when it was compared with the PC12 (just added NGF followed by starvation).
I have found some black spots attached on my cells (neural spheres) !!
Can anyone tell me what has happened ? The pictures are as follows :