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Cell frozen - (Dec/10/2006 )

I'd like to freeze cells and medium together before adding lysis buffer and scraping cell. But I wonder if I can still get intact cells after that? Would cells break and proteins leak to medium?
Many thanks!

-josophen-

No, your cells will break and their content will leak out. People usually remove the old medium and wash 1-3 times with PBS, then lyse the cells.

-genehunter-1-