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medium -preparation for CD34 cells - (May/29/2007 )


I want to prepare a new medium for CD34 cells, instead to buy.
Please any advice, what is it important for the praparation such medium. I have'nt any autoclave, and other devices for sterilisation process, because our labor is too little for that.
We are working with single packaged sterile pipettes, etc.

please help me mellow.gif


you may filter using a 0.22 μm filter (Millipore for example)


I agree with Pumuki, a 0.22um millipore filter is what we use to sterilise our tissue culture media. You shouldn't autoclave tissue culture media anyways.