Has anyone used 8E5 cells as standard for HIV qPCR? - HIV standards (Nov/18/2005 )

Does anyone have experience with using 8E5 cells (which are supposed to have 1 copy HIV per cell) as a quantitation standard?

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I join Ticklish Bob in requesting for assistance wrt 8E5 cells.....why am I constantly counting up to 3 proviral copies per 8e5 cells...what am I doing wrong

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