Cell surface Protein Isolation - Protein extraction procedure (Oct/16/2008 )

Hi all,

I am trying to study cell surface proteins and was wanting to do westerns. I would like to try extracting cell surface proteins without buying an expensive kit.

Has anyone tried this in the lab and can you share this information.

thanks

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tyou can try to immunoisolate or biotinylate the surface proteins with ectodomains...

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yeah, but that would also be expensive!

I know Qiagen has a kit that separates 4 cell fractions;...but you don't want to use kits

have a look at Sambrook's handbook, I remember reading a method there.

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thanks,

I will try the Molecular cloning book first. I hope it works .....

Pooja

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Isolate the PM by differentiate centrifugation, then using detergent to extract proteins. Search PubMed for differentiate centrifugation

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