# growth curve? - how to establish growth curve of specific cell line.... (Sep/23/2008 )

hi

can any one help me how can i find or generate good growth curve of a specific cell line??????how to find cell line exponential phase????also tell me calculation with protocol

im confused

THANKS

-student in search-

QUOTE (student in search @ Sep 23 2008, 02:06 AM)

hi

can any one help me how can i find or generate good growth curve of a specific cell line??????how to find cell line exponential phase????also tell me calculation with protocol

im confused

THANKS

can any one help me how can i find or generate good growth curve of a specific cell line??????how to find cell line exponential phase????also tell me calculation with protocol

im confused

THANKS

I take it you know how to count cells?

So, seed a several wells of a plate with cells at a known density (try 10,000 cells/cm

^{2}, and then lift and count the number of cells per volume of harvest over a set time course (e.g. every 2 days harvest a well), work out how many cells you have in total and plot those numbers on a time vs cell number graph. Exponential phase is where cell numbers are doubling or more per unit time, plotting on a log-linear scale will help identify it as it will become straight rather than curved.

Calculation for what? If for the curve, try some regression analysis, or get a graphing program (not Excel, it is terrible) and use that to tell you.

-bob1-