Treatment of cancer cell - (Oct/02/2006 )
As for your information, I had treated the cell with a natural compound in the 96-well plate. I able to get the IC50 and the morphological changes of the treated cancer cell also can be observed in which i noticed that the treated cell round up as compare to the untreated cell.
However when i treated the cell in the 6-well plate,not morphological changes can be observed after the adding of the natural compound. The concentration of compound that i add in the 6-well plate is the same as in the 96-well plate.
Can anyone give me some suggestion?
Many many thanks.
May be due to the cell density.
ie. the cell density of the 96 well plates is lower than the 6 well plates.
Hope this may help.
as suggested cell density
or different plate material (PET? PC? coated/uncoated?)
or different volumes of medium which influence diffusion of O2 and CO2 to the cells