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Cotransfection of DNA and siRNA in Neuro2a cells - (Sep/16/2008 )

Hi all, does anyone can help me to how to perform co-transfection of DNA and siRNA in Neuro2a cells? I performed cotransfection by using Lipofectamine 2000 and DharmaFect Duo reagent, followed the manufacturer protocol, my cells are died after 24 hours? Do I need to seed alot of cells before the transfection? What is the perfect way to perform cotransfection so that the efficiency of siRNA knockdown is good? Thanks

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i know with lipofectamine, you do need a lot of cells to reduce toxicity of lipofectamine itself. I do not have an idea about dharmafect.

You need the right siRNA sequence to have good knockdown.