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Fixation of cell culture with Formaldehyde - need protocol (May/12/2005 )

does enyone knows a specific protocol for Fixation of cell cultures with Formaldehyde for cell cycle analysis?



i din't use formaldehyde to fix cells for cell cycle analysis. you could fix your cells with 70% ethanol overnight at 4 centigrade. it works well.


What kind of cell cycle analyses? Flow based with PI?



i use formaldehyde fixation in DAPI staining and nick translation assay (4% formaldehyde in PBS for 10 min and 15 min, respectively), but these are apoptosis assays.

for cell cycle analysis i use PI staining and flow.