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pls recommend a serum independent expression promoter for mammalian cells - (Dec/09/2008 )


I want to construct a stable cell line expressing the gene of interest. However, the following assay will be performed in serum withdrawl condition. I learned that the CMV promoter is serum dependent and lacking of serum in the culture medium will slience gene expression. Does anyone can recommend a serum independent promoter for mammalian cells?

Thanks a lot!



does anyone can help?