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Ways to stop the cell at G1 phase? - (Dec/04/2008 )


I am new to cell biology. huh.gif

I just come across some experiments that need to stop the cells (Hela cells) at G1 phase. Actually what are the ways to achieve this?

Thanks a lot!


hi cityugrad i think if you remove the serum from your media the cells will stop in G1 phase


Serum starvation is the most common way to do this, however you will get a small population of cells that will stop in other parts of the cell cycle. There are a few specific cell cycle inhibitors about that can do this. Sigma has a cell cycle regulators page that will supply you with the info you need.