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Single-cell RT-PCR with only ~10-100 Cells - (Sep/19/2001 )

Hi Mathias,

the RNeasy Kit from Qiagen is supposed to yield enough RNA from about 100 cellsGood luck


hi,i must identify mRNA from small frozen Tissue Samples isolated by Laser Microsdissection.I need a protocol of isolation RNA (-->RT-PCR) from frozen Tissue Samples. The samples contain only 10- 100cells!!!!!

-Mathias Droll-

Maybe you could see "RT-PCR WITHOUT RNA ISOLATION", Klebe et al, BioTechniques 21: 1094-1100(Dec 1996). They report successful RT-PCR from less than 250 cells.