competent cell - (Mar/18/2006 )
I have to prepare competent cell of srain E.coli K12 , TB1 . can i prepare the competent cell for this strain through the same protocol which is used for DH5-Alpha. Is it will work or not.
if no then which protocol is used for this strain.
best regards
-anu1-
Yes, you can generally make any E. coli strain competent with the same protocol. What method do you use?
-HomeBrew-
I used CaCl2 method. Wasing twice with just CaCl2 first then CaCl2 and glycerol.
I used this method for DH5-alpha so it can be used for the E.Coli K12, TB1
.
Best regards
QUOTE (HomeBrew @ Mar 18 2006, 07:33 PM)
Yes, you can generally make any E. coli strain competent with the same protocol. What method do you use?
-anu1-