FBS or FCS for NIH3T3 cells - How to grow these cells optimal (Aug/23/2006 )
Just a quick question.
Anyone experienced with NIH3T3 cells?
I know they should be easy to grow and so on...but ATCC recommends to use FCS instead of FBS for culture.
Can anybody agree or disagree on that? Just don`t want to order the stuff when I don`t have too... .
Thanks al lot!
fetal calf serum instead of fetal bovine serum?
I thought that it was the same thing?
They recommend Calf serum !
i.e. not fetal !
I'm sorry for this post, but I was so surprised,and thought that I missed something for years
I never cultured NIH3T3, I can't give you an advice.
yeah true...sorry, posted it wrong..it`s meant to be calf serum... .
So anybody else?
NIH3T3 cells grow like weeds and I have used both FCS and FBS. The source of your serum is however much more important :-
i.e. British £20
South American £50
New Zealand £250
And guess what the quality of the serum is in the same descending order as the price. In your case the NIH3T3 cells are not a fastidious line and do not require any great looking after.
FCS and FBS are the same thing. FCS is a misnomer.
My experience has been that the NIH3T3 cells grow better in BCS (Bovine Calf Serum) than they do in FBS. Both, growth rate and maximum cell density are higher in BCS.
I used 3T3's in my dissertation, they grow like crazy. I used IMDM - 10%BCS, I tried FBS but they grew slower. You also have to remember that they are extremely contact inhibited, and once confluent lose viability quickly. Good luck.
I used NIH 3T3 and I always cultured them in Calf serum