Experiment with KATO III cells - (Sep/12/2007 )
Hi everybody,
I want to do an experiment with KATO-III cells, but the cells are both in suspension and attached (loosely) in the same culture. What is the best way to seed the cells and stimulate? I need the supernatant for my experiments.
I myself was thinking of Poly-L-lysin coated plates (maybe the cells in suspension will attach), but I can nowhere find a reference with KATO-III cells involved.
Who did this with KATO-cells?
Thanks in advance.
-malooney-
QUOTE (malooney @ Sep 12 2007, 02:29 AM)
Hi everybody,
I want to do an experiment with KATO-III cells, but the cells are both in suspension and attached (loosely) in the same culture. What is the best way to seed the cells and stimulate? I need the supernatant for my experiments.
I myself was thinking of Poly-L-lysin coated plates (maybe the cells in suspension will attach), but I can nowhere find a reference with KATO-III cells involved.
Who did this with KATO-cells?
Thanks in advance.
I want to do an experiment with KATO-III cells, but the cells are both in suspension and attached (loosely) in the same culture. What is the best way to seed the cells and stimulate? I need the supernatant for my experiments.
I myself was thinking of Poly-L-lysin coated plates (maybe the cells in suspension will attach), but I can nowhere find a reference with KATO-III cells involved.
Who did this with KATO-cells?
Thanks in advance.
KATOIII are known to be cultured attached, suspended or mixed; poly-lysine may help to increase adherence but uncoated plating should do; KATOIII need high complementation with 20% FCS; trypsination is short with 5 -10 min
-The Bearer-